The Italian side won 3-0 away in Bialystok, with goals from Luca Ranieri, Rolando Mandragora and Roberto Piccoli. The victory secures a place in the Conference League last‑16.
Jagiellonia were without key players including Afimico Pululu and Taras Romanczuk, while Fiorentina fielded a squad missing David De Gea, Moise Kean and others. Luca Lezzerini made his second UEFA appearance.